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transblot sd cell semidry transfer machine  (Bio-Rad)


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    Structured Review

    Bio-Rad transblot sd cell semidry transfer machine
    Transblot Sd Cell Semidry Transfer Machine, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3986 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transblot+sd+cell+semidry+transfer+machine/Trans-Blot+SD/pm24672800-52-26-32
    Average 99 stars, based on 3986 article reviews
    transblot sd cell semidry transfer machine - by Bioz Stars, 2026-09
    99/100 stars

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    Electrophoresis:

    Article Title: The role of PinX1 in growth control of breast cancer cells and its potential molecular mechanism by mRNA and lncRNA expression profiles screening.
    Article Snippet: Total proteins from all the cell samples were extracted using RIPA buffer containing 1mM PMSF (Beyotime, Haimen, China). .. 50μg of each protein was loaded on 10% SDS polyacrylamide gels for electrophoresis, and then the proteins were transferred to PVDF membranes (Millipore, USA) by a Transblot SD Cell semidry transfer machine (Bio Rad, USA). .. After being blocked by 5% nonfat milk, the membranes were incubated with goat anti-PinX1 antibody (1 : 500, Santa Cruz Biotechnology, Santa Cruz, USA) and HRP conjugated rabbit anti-goat IgG (1 : 5000, Multisciences, Hangzhou, China) for the detection of PinX1 protein. β-actin was detected by using the mouse anti-βactin antibody (1 : 2000, Multisciences, Hangzhou, China) and HRP conjugated goat anti-mouse IgG antibody (1 : 5000, Multisciences, Hangzhou, China).

    Article Title: The Role of PinX1 in Growth Control of Breast Cancer Cells and Its Potential Molecular Mechanism by mRNA and lncRNA Expression Profiles Screening
    Article Snippet: Total proteins from all the cell samples were extracted using RIPA buffer containing 1 mM PMSF (Beyotime, Haimen, China). .. 50 μ g of each protein was loaded on 10% SDS polyacrylamide gels for electrophoresis, and then the proteins were transferred to PVDF membranes (Millipore, USA) by a Transblot SD Cell semidry transfer machine (Bio Rad, USA). .. After being blocked by 5% nonfat milk, the membranes were incubated with goat anti-PinX1 antibody (1 : 500, Santa Cruz Biotechnology, Santa Cruz, USA) and HRP conjugated rabbit anti-goat IgG (1 : 5000, Multisciences, Hangzhou, China) for the detection of PinX1 protein. β -actin was detected by using the mouse anti- β -actin antibody (1 : 2000, Multisciences, Hangzhou, China) and HRP conjugated goat anti-mouse IgG antibody (1 : 5000, Multisciences, Hangzhou, China).



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    99
    Bio-Rad transblot sd cell semidry transfer machine
    Transblot Sd Cell Semidry Transfer Machine, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transblot+sd+cell+semidry+transfer+machine/Trans-Blot+SD/pm24672800-52-26-32
    Average 99 stars, based on 1 article reviews
    transblot sd cell semidry transfer machine - by Bioz Stars, 2026-09
    99/100 stars
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